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KMID : 1134820170460121568
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Journal of the Korean Society of Food Science and Nutrition
2017 Volume.46 No. 12 p.1568 ~ p.1573
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Optimization of β-1,3/1,6-Glucan Production of Aureobasidium pullulans KSY-0516 Strain
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Kim Min-Soo
Kim Joong-Su
Ryu Min-Jeong
Oh Sung-Hoon
Kim Ki-Hong
Hwang Kwon-Tack
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Abstract
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β-Glucans are major bioactive compounds known to have biological activities, including anti-cancer, anti-inflammatory, and immune-modulating properties. Aureobasidium pullulans, known as black yeast, produces a variety of black β-glucans, which can be used to produce white β-1,3/1,6-glucan using a mutation technique. The purpose of this study was to determine the optimum production yield of β-1,3/1,6-glucan using response surface methodology, which is a statistical procedure used for optimization studies. A central composite design was applied to investigate the effects of independent variables, sucrose concentration (X1), KNO3 concentration (X2), and oxalic acid concentration (X3) on responses such as yield and β-1,3/1,6-glucan production rate. A maximum yield of 1.86217 g/L was obtained at 4.74% sucrose, 0.19% KNO3, and 0.036% oxalic acid. Based on the above results, the optimal production and efficient production of β-glucans can be achieved.
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KEYWORD
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response surface methodology, central composite design, β-1,3/1,6-glucan, optimization
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